Apoptotic cell death is fundamental cell process that utilizes the cell death receptor family signaling network. Caspases are activated by a cleavage that occurs adjacent to an aspartate; this cleavage liberates the individual units and allows formation of an a2b2 tetramer. …
How does caspase-3 work?
Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular structures such as DNA fragmentation or degradation of cytoskeletal proteins (1). The activity of caspase-3 is tightly regulated and it is produced as zymogen in an inactive pro-form (1).
What do caspases do?
Caspases (cysteine-aspartic proteases) are proteolytic enzymes largely known for their role in controlling cell death and inflammation. Based on their function, mammalian caspase-2, -3, -7, -8, -9 and -10 are apoptotic caspases, where as caspase-1, -4, -5, -11 and -12 are involved in inflammation.
Why is caspase-3 important?
Caspases are crucial mediators of programmed cell death (apoptosis). Thus, caspase-3 is essential for certain processes associated with the dismantling of the cell and the formation of apoptotic bodies, but it may also function before or at the stage when commitment to loss of cell viability is made.
Where does caspase 3 activation occur?
apoptotic cell
Activation. Caspase-3 is activated in the apoptotic cell both by extrinsic (death ligand) and intrinsic (mitochondrial) pathways. The zymogen feature of caspase-3 is necessary because if unregulated, caspase activity would kill cells indiscriminately.
How are executioner caspases activated?
Initiator caspases are activated by intrinsic and extrinsic apoptopic pathways. This leads to the activation of other caspases including executioner caspases that carry out apoptosis by cleaving cellular components.
Is cleaved caspase 3 active?
The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).
What is caspase-3 antibody used for?
Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa). Species Reactivity: Human, Mouse, Rat, Monkey. Species predicted to react based on 100% sequence homology: Pig
What is the difference between caspase-3 and α-tubulin Mab?
Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA.
Is there a CASP3 antibody for HCT116 cells?
The absence of signal in the CASP3 knock-out HCT116 cells confirms specificity of the antibody for CASP3. Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), and NIH/3T3 cells, untreated or staurosporine-treated (1uM, 3hrs), using Caspase-3 Antibody.
How is cleaved caspase-3 treated in Jurkat cells?
Immunoprecipitation of cleaved caspase-3 from Jurkat cell extracts untreated (control) or treated with etoposide (25uM 5hrs) (apoptotic) using Caspase-3 (8G10) Rabbit mAb, and western probed with the same antibody.
