Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide.
Does GDF-5 promote chondrogenesis?
Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).
Does recombinant human GDF-5 induce alkaline phosphatase production?
Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED 50 for this effect is 0.2-1.2 μg/mL.
What is the half-life of recombinant or natural proteins in culture?
R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.
